Colecystokinin octapeptide CCK‐8 and carbachol reduce [32P]orthophosphate labeling of phosphatidylcholine without modifying phospholipase D activity in rat pancreatic acini
- 30 November 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 486 (1) , 63-67
- https://doi.org/10.1016/s0014-5793(00)02233-x
Abstract
We have studied phospholipase D activation in [32P]orthophosphoric acid‐prelabeled rat pancreatic acini by measuring the formation of 32P‐phosphatidylalcohols as stimulated in the presence of ethanol or butanol. A small but significant and time‐dependent basal accumulation of [32P]phosphatidylethanol and [32P]phosphatidylbutanol was detected, which was further stimulated by phorbol myristate acetate, orthovanadate and pervanadate. However, the secretagogues cholecystokinin octapeptide and carbachol did not enhance basal accumulation of 32P‐phosphatidylalcohol, yet they decreased [32P]phosphatidylcholine content and stimulated the generation of [32P]phosphatidic acid. Our results stress the need to examine the transphosphatidylation reaction as well as agonist effects on the synthesis of phosphatidylcholine in order to assess unambiguously phospholipase D activity.Keywords
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