A Sensitive High Performance Liquid Chromatographic Method for U-80, 278A, A Substituted Aminotetralin, in Rat Plasma, Whole Blood and Brain Tissue
- 1 December 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 14 (20) , 3761-3782
- https://doi.org/10.1080/01483919108049493
Abstract
A sensitive analytical method for U-80, 278A, a substituted aminotetralin analogue in rat plasma, whole blood and brain tissue has been developed. The method involves solid phase extraction, efficient reversed phase HPLC and fluorescence detection, and can measure 1 ng/ml from 50 μl samples. During method development, many analogues were investigated and a wide range of extraction and HPLC conditions were explored. These experiments enabled rapid modification and revalidation of the method to support animal experiments with novel analogues.Keywords
This publication has 1 reference indexed in Scilit:
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