Effect of Temperature-Sensitive Mutation on Activity of the RNA Transcriptase of Vesicular Stomatitis Virus New Jersey
- 1 June 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 30 (3) , 692-700
- https://doi.org/10.1128/jvi.30.3.692-700.1979
Abstract
The virion-associated RNA transcriptase activity of vesicular stomatitis virus New Jersey temperature-sensitive (ts) mutants was assayed in vitro at the permissive (31.degree. C) and restrictive (39.degree. C) temperatures. RNA synthesis at 39.degree. C by the RNA-negative ts A1 and the RNA-positive ts C1 and ts D1 mutants was similar to that of wild-type virus. The RNA-negative ts B1 synthesized only small amounts of RNA in vitro at 39.degree. C. The 3 mutants of complementation group E were dissimilar in the amounts of RNA they synthesized at 39.degree. C: ts E1 synthesized very little RNA, ts E2 synthesized moderate amounts, and RNA synthesis by ts E3 was not inhibited. The 2 mutants of group F were also dissimilar, since ts F1 synthesized very little RNA at 39.degree. C, whereas ts F2 synthesized as much RNA as wild-type virus. The revertant clones ts B1/R1, ts E1/R1, and ts F1/R1 synthesized RNA at 39.degree. C in amounts comparable to wild-type virus, indicating that the heat sensitivity of the transcriptase activity of the mutants ts B1, ts E1 and ts F1 was associated with temperature sensitivity. Similar heat sensitivities were observed when transcribing nucleoprotein complexes were used in the assays, showing that the mutated polypeptides were part of the viral core. The heat stability of the mutant ts B1 was similar to that of wild-type virus, and in vitro RNA synthesis was fully restored when the temperature was lowered to 31.degree. C after 30 min of preincubation at 39.degree. C, showing that the inhibition was due to reversible configurational change of the mutated polypeptide. When virions of the mutant ts E1 were heated for 5 h at 39.degree. C, their infectivity and transcriptase activity were as stable as those of the wild-type virus, whereas transcriptase activity became very heat labile after disruption of the viral coat with a neutral detergent. This suggests an interaction between the mutated polypeptide and a coat polypeptide which stablizes the activity of the transcriptase. The RNA transcriptase activity of the mutant ts F1 was also heat labile although to a lesser extent than that of ts E1. Thus, the defects in transcriptase activity of groups B, E and F suggest that all 3 polypeptides of the virus core, polypeptides L, N and NS, are involved in the transcription. The mutated gene products of groups E and F are probably multifunctional, being required both in transcription and replication, and the gene product of group E may also be involved in some late stage of virus development.This publication has 27 references indexed in Scilit:
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