Calcium binding by rat submaxillary glands in vitro

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Abstract
The binding of Ca by rat-submaxillary-gland homogenates in vitro was completely reversible in the presence of sodium chloride at an ionic strength of 0. 15. The increase in calcium bound by slices or homogenates was approximately log-linear over the range 1-10 mM-Ca2+. Slices or homogenates of rat submaxillary glands bind approximately 3 times as much Ca as do serum proteins at the same ionic strength. The range of temperature from 2[degree] to 30[degree] had little effect on Ca binding by rat-submaxillary -gland slices or homogenates. Change in pH from 7.4 to 5.0 decreased the amount of Ca bound by rat-submaxillary-gland homogenates to approximately one-half the initial amount, whereas increase of pH from 7. 4 to 8. 5 did not increase significantly the amount of Ca bound.