Viral immunoblotting: A sensitive method for detecting viral-specific oliogoclonal bands in unconcentrated cerebrospinal fluid
- 1 June 1984
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 4 (6) , 505-510
- https://doi.org/10.1007/bf01122226
Abstract
A new method for detecting viral antibodies in cerebrospinal fluid is described. The technique has many advantages over previously published methods in that it is highly sensitive eliminating the need to concentrate the CSF, takes 5 h to complete, avoids the use of radionucleides, and most importantly circumvents problems associated with prozone effects which occur in immunoprecipitation reaction since the viral antigen is immobilized on nitrocellulose membranes.This publication has 11 references indexed in Scilit:
- A rapid method for detecting oligoclonal IgG in unconcentrated CSF, by agarose isoelectric focusing, transfer to cellulose nitrate and immunoperoxidase stainingJournal of Neuroimmunology, 1983
- Cerebrospinal fluid IgG bands and virus-specific IgG, IgM, and IgA antibodies in herpes simplex virus encephalitisJournal of Neuroimmunology, 1982
- Herpes simplex virus encephalitis: Intrathecal synthesis of oligoclonal virus-specific IgG, IgA and IgM antibodiesZeitschrift für Neurologie, 1982
- The Wellcome Foundation Lecture, 1980 - Monoclonal antibodies from hybrid myelomasProceedings of the Royal Society of London. B. Biological Sciences, 1981
- Antibodies to viral and non-viral antigens in subacute sclerosing panencephalitis and multiple sclerosis demonstrated by thin-layer polyacrylamide gel isoelectric focusing, antigen immunofixation and autoradiographyJournal of the Neurological Sciences, 1981
- The use and abuse of the cerebrospinal fluid IgG profile in the adult: A practical evaluationAnnals of Neurology, 1980
- Multiple Sclerosis: Local Synthesis of Electrophoretically Restricted Measles, Rubella, Mumps and Herpes Simplex Virus Antibodies in the Central Nervous SystemScandinavian Journal of Immunology, 1978
- Demonstration of Electrophoretically Restricted Virus‐Specific Antibodies in Serum and Cerebrospinal Fluid by Imprint ElectroimmunofixationScandinavian Journal of Immunology, 1978
- Mumps Meningitis: Prolonged Pleocytosis and Occurrence of Mumps Virus-Specific Oligoclonal IgG in the Cerebrospinal FluidEuropean Neurology, 1978
- Oligoclonal Measles Virus‐Specific IgG Antibodies Isolated from Cerebrospinal Fluids, Brain Extracts, and Sera from Patients with Subacute Sclerosing Panencephalitis and Multiple SclerosisScandinavian Journal of Immunology, 1976