Detection of Superoxide Free Radicals in Rats with Acute Pancreatitis
- 1 August 1995
- journal article
- research article
- Published by Wolters Kluwer Health in Pancreas
- Vol. 11 (2) , 122-126
- https://doi.org/10.1097/00006676-199508000-00002
Abstract
To study the importance of oxygen-derived free radicals in acute pancreatitis, an experimental study of in vivo detection of superoxide free radicals (O2−) was performed using rats. Using a new chemiluminescence probe (2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazol[1,2-1]pyrazin-3-one; MCLA; a Cypridina luciferin analogue) and a highly sensitive photon counting system, O2− from the pancreatic surface of rats with experimental acute pancreatitis induced by 180 μg cedein/kg was detected. The time course of MCLA-dependent luminescence suggested that O2− production began 2–3 h after cerulein injection and then decreased gradually. Superoxide free radical production in the pancreas of rats with cerulein-induced acute pancreatitis was confirmed using MCLA-dependent chemiluminescence. This new method allows direct observation of the behavior of oxygen-derived free radicals.Keywords
This publication has 0 references indexed in Scilit: