Isolation and characterisation of a yeast chromosomal replicator
- 1 November 1979
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 282 (5734) , 39-43
- https://doi.org/10.1038/282039a0
Abstract
A yeast [Saccharomyces cerevisiae] DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850 base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.Keywords
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