Isolation of competition-defective mutants of Rhizobium fredii
Open Access
- 31 December 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 169 (1) , 410-413
- https://doi.org/10.1128/jb.169.1.410-413.1987
Abstract
We coupled Tn5 mutagenesis with a competition assay to isolate mutants of Rhizobium fredii USDA 257 that are defective in competition for nodulation of soybeans. Two mutants with single Tn5 inserts in the chromosome showed reduced competitiveness in vermiculite but were identical to the wild-type strain in symbiotic properties when inoculated alone. Recombination of Tn5 and flanking genomic regions cloned from the mutants into the parent strain showed that Tn5 was responsible for the mutant phenotype.This publication has 24 references indexed in Scilit:
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