Detection of K-ras mutation in sputum by mutant-allele-specific amplification (MASA)
- 1 January 1993
- journal article
- method
- Published by Hindawi Limited in Human Mutation
- Vol. 2 (2) , 112-117
- https://doi.org/10.1002/humu.1380020209
Abstract
From 10 to 30% of lung carcinomas examined to date contain mutant K-ras genes. We report here that the mutant-allele-specific amplification (MASA) method may be useful for detection of the K-ras mutations in cells obtained from the sputum of patients with lung cancer. The PCR product from one of five patients revealed an alteration when mixed oligonucleotides representing variants of the second letter at codon 12 of this gene were used as 5′ primers, and further experiments showed a mutation of GGT (Gly) to GAT (Asp)at codon 12. The MASA system could also be applied to an examination of metastatic lung carcinomas, particularly from adenocarcinomas in colon and pancreas in which frequent K-ras mutations are detected, and to mass-screening for colorectal tumors using DNA isolated from feces as template.Keywords
This publication has 15 references indexed in Scilit:
- Mutations of Chromosome 5q21 Genes in FAP and Colorectal Cancer PatientsScience, 1991
- p53 Mutations in Human CancersScience, 1991
- Mutations in the p53 gene occur in diverse human tumour typesNature, 1989
- Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reactionGenomics, 1989
- High frequency of Ki‐ras codon 12 mutations in pancreatic adenocarcinomasInternational Journal of Cancer, 1989
- Abnormalities in Structure and Expression of the Human Retinoblastoma Gene in SCLCScience, 1988
- Mutational Activation of the K-rasOncogeneNew England Journal of Medicine, 1987
- Detection of high incidence of K-ras oncogenes during human colon tumorigenesisNature, 1987
- Prevalence of ras gene mutations in human colorectal cancersNature, 1987
- Detection of single base substitutions in total genomic DNANature, 1985