Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Clinically Important Yeast Species
- 1 October 2010
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 48 (10) , 3482-3486
- https://doi.org/10.1128/jcm.00687-09
Abstract
We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of yeast species. Using Bruker Daltonics MALDI BioTyper software, we created a spectral database library with m/z ratios of 2,000 to 20,000 Da for 109 type and reference strains of yeast (44 species in 8 genera). The database was tested for accuracy by use of 194 clinical isolates (23 species in 6 genera). A total of 192 (99.0%) of the clinical isolates were identified accurately by MALDI-TOF MS. The MALDI-TOF MS-based method was found to be reproducible and accurate, with low consumable costs and minimal preparation time.Keywords
This publication has 30 references indexed in Scilit:
- High-Throughput Identification of Bacteria and Yeast by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Conventional Medical Microbiology LaboratoriesJournal of Clinical Microbiology, 2010
- Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Fast and Reliable Identification of Clinical Yeast IsolatesJournal of Clinical Microbiology, 2009
- Candidaemia with uncommon Candida species: predisposing factors, outcome, antifungal susceptibility, and implications for managementClinical Microbiology & Infection, 2009
- Results from the ARTEMIS DISK Global Antifungal Surveillance Study, 1997 to 2007: 10.5-Year Analysis of Susceptibilities of Noncandidal Yeast Species to Fluconazole and Voriconazole Determined by CLSI Standardized Disk Diffusion TestingJournal of Clinical Microbiology, 2009
- Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cystic Fibrosis PatientsJournal of Clinical Microbiology, 2008
- Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting BacteriaJournal of Clinical Microbiology, 2008
- Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related speciesEuropean Journal of Clinical Microbiology & Infectious Diseases, 2008
- Evaluation of VITEK 2 and RapID Yeast Plus Systems for Yeast Species Identification: Experience at a Large Clinical Microbiology LaboratoryJournal of Clinical Microbiology, 2007
- Development and Clinical Application of a Panfungal PCR Assay To Detect and Identify Fungal DNA in Tissue SpecimensJournal of Clinical Microbiology, 2007
- Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass SpectrometryJournal of Clinical Microbiology, 2006