Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly

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Abstract
The SV-40 virion assembly process was studied with pulse-labeling kinetics of virion proteins, CsCl gradient analysis, EM and low-salt gel electrophoresis. The results are consistent with the model of gradual addition and organization of capsid proteins around SV-40 chromatin. Empty virions, as observed in the CsCl gradient by previous workers, were the dissociation product of immature virus. Histone H1 was found in SV-40 chromatin and virion assembly intermediates but not in the mature virion banding at 1.34 g/ml in the CsCl gradient. [African green monkey kidney CV-1 cells were used.].