Quantitation of Immunoadsorbed Flavoprotein Oxidases by Luminol-Mediated Chemiluminescence

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Abstract
The detection of the flavoenzymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase at the sub-femtomol level was achieved by coupling the reaction of the immunoadsorbed proteins to the peroxidase-catalyzed oxidation of luminol. The H2O2-producing oxidases retained their full activity when bound to the respective immobilized antibodies. This fact allowed the concentration of the enzymes from very dilute solutions and the quantitative assay of their activities in the .mu.U range. Due to strict stereoselectivity and the absence of immunological cross-reactivity, the 2 flavoproteins could be determined in the same solution. This method was used to measure the 6-hydroxy-D-nicotine oxidase and 6-hydroxy-L-nicotine oxidase activities in Escherichia coli RR1 and different Arthrobacter strains cultured under non-inducing conditions. The same activity ratio of 6-hydroxy-L-nicotine oxidase/6-hydroxy-D-nicotine oxidase as in DL-nicotine-induced cells of A. oxidans was observed in non-induced wild-type and in riboflavin-requiring (rf-) mutant cells of this aerobe.