The cystine and methionine in certain species of grassland herbage

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Abstract
Protein prepns. made from 63 samples of Trijolium repens, Dactylis glomerata, Lolium perenne and Phleum pratense by extraction with a buffer soln. of pH 9.2 and precipitation at pH 4.5 contained on the average 11% N which accounted for 15-43% of the total N in the plants. The distribution of S was estimated by hydrolysis with HI followed by detn. of the cysteine and homocysteine in the digest and of CH3I and inorg. S by absorption. The av. values as % N in the proteins were 1.16 for cystine and 1.35 and 1.03 for methionine by the CH3I and homocysteine methods resp. They were almost independent of species, site and manuring but were significantly greater in samples which had reached flowering stage than in samples of young or aftermath or grazed grass.

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