Quantitation of Glycosylated Hemoglobin: Elimination of Labile Glycohemoglobin During Sample Hemolysis at pH 5

1 July 1982

journal article
Published by American Diabetes Association in Diabetes

Vol. 31 (7) , 630-633
https://doi.org/10.2337/diab.31.7.630

Abstract

A simple method for the elimination of labile glycohemoglobin in the chromatographic quantitation of glycosylated hemoglobin is described. Use is made of the instability of Schiff base adducts in acidic solution. Erythrocytes are lysed with a pH 5 buffer. At this pH dissociation reaches completion during sample preparation.

Keywords

LABILE GLYCOHEMOGLOBIN
GLYCOSYLATED HEMOGLOBIN
CHROMATOGRAPHIC QUANTITATION
SCHIFF BASE
DISSOCIATION REACHES
PH DISSOCIATION
SIMPLE METHOD

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