Heat‐labile uracil‐DNA glycosylase: purification and characterization
- 10 June 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 388 (1) , 1-4
- https://doi.org/10.1016/0014-5793(96)00444-9
Abstract
A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23 400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40°C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.Keywords
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