Re‐evaluating the role of strongly charged sequences in amphipathic cell‐penetrating peptides
- 26 July 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (20) , 4498-4502
- https://doi.org/10.1016/j.febslet.2005.06.085
Abstract
Cell-penetrating peptides (CPPs) are able to translocate across biological membranes and deliver bioactive proteins. Cellular uptake and intracellular distribution of CPPs is commonly evaluated with fluorescent labels, which can alter peptide properties. The effect of carboxyfluorescein label in the Lys-rich domain of the amphipathic CPP pep-1, was evaluated and compared with non-labelled pep-1 in vitro and in vivo. A reduced membrane affinity and an endosomal-dependent translocation mechanism, at variance with non-labelled pep-1, were detected. Therefore, the charged domain is not a mere enabler of peptide adsorption but has a crucial role in the translocation pathway of non-labelled pep-1.Keywords
This publication has 19 references indexed in Scilit:
- Translocation of β-Galactosidase Mediated by the Cell-Penetrating Peptide Pep-1 into Lipid Vesicles and Human HeLa Cells Is Driven by Membrane Electrostatic PotentialBiochemistry, 2005
- Environmental factors that enhance the action of the cell penetrating peptide pep-1Biochimica et Biophysica Acta (BBA) - Biomembranes, 2005
- Consequences of Nonlytic Membrane Perturbation to the Translocation of the Cell Penetrating Peptide Pep-1 in Lipidic VesiclesBiochemistry, 2004
- Uptake of analogs of penetratin, Tat(48–60) and oligoarginine in live cellsBiochemical and Biophysical Research Communications, 2003
- Using a Novel Dual Fluorescence Quenching Assay for Measurement of Tryptophan Depth within Lipid Bilayers To Determine Hydrophobic α-Helix Locations within MembranesBiochemistry, 2003
- Cell-penetrating PeptidesJournal of Biological Chemistry, 2003
- Taking the Cell by Stealth or Storm? Protein Transduction Domains (PTDs) as Versatile Vectors for DeliveryDNA and Cell Biology, 2002
- Up-regulation of Sodium-dependent Glucose Transporter by Interaction with Heat Shock Protein 70Published by Elsevier ,2002
- Tryptophan fluorescence study of the interaction of penetratin peptides with model membranesEuropean Journal of Biochemistry, 2002
- The Inhibitory Role of DOC-2/DAB2 in Growth Factor Receptor-mediated Signal CascadePublished by Elsevier ,2001