Generation Of Transgenic Dairy Cattle Using ‘in vitro’ Embryo Production
- 1 September 1991
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 9 (9) , 844-847
- https://doi.org/10.1038/nbt0991-844
Abstract
We have combined gene transfer, by microinjection, with ‘in vitro’ embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.Keywords
This publication has 23 references indexed in Scilit:
- Bovine in vitro fertilization with frozen-thawed semenPublished by Elsevier ,2003
- Some factors affecting the efficacy of oviduct tissue-conditioned medium for the culture of early bovine embryosReproduction, 1991
- Genetransfer by pronuclear injection in the bovineTheriogenology, 1988
- Alteration of the quality of milk by expression of sheep β-lactoglobulin in transgenic miceNature, 1987
- Culture of one- and two-cell bovine embryos to the blastocyst stage in the ovine oviductTheriogenology, 1987
- Production of transgenic mice, rabbits and pigs by microinjection into pronucleiReproduction in Domestic Animals, 1985
- Estimation of embryonic losses in bovine embryo transfer recipients from progesterone profiles and returns to estrusTheriogenology, 1985
- Use of the rabbit oviduct as a screening tool for the viability of mammalian eggsTheriogenology, 1984
- No seasonal effect on embryo donor performance in the southwest region of the USATheriogenology, 1984
- Bovine embryo morphology and evaluationTheriogenology, 1983