A leucine‐rich repeat protein of carrot that exhibits antifreeze activity
Open Access
- 26 March 1999
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 447 (2-3) , 171-178
- https://doi.org/10.1016/s0014-5793(99)00280-x
Abstract
A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine‐rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs.Keywords
This publication has 55 references indexed in Scilit:
- The Tomato Cf-5 Disease Resistance Gene and Six Homologs Show Pronounced Allelic Variation in Leucine-Rich Repeat Copy NumberPlant Cell, 1998
- Structural diversity of leucine-rich repeat proteins 1 1Edited by F. CohenJournal of Molecular Biology, 1998
- Structural basis for the binding of a globular antifreeze protein to iceNature, 1996
- Plant disease resistance genes: structure, function and evolutionCurrent Opinion in Biotechnology, 1996
- Purification and characterization of a thermal hysteresis protein from a plant, the bittersweet nightshade Solanum dulcamaraBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1994
- Plant thermal hysteresis proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Nuclear DNA content of some important plant speciesPlant Molecular Biology Reporter, 1991
- How signal sequences maintain cleavage specificityJournal of Molecular Biology, 1984
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962