Effect of Phytohemagglutinin on Expression of Human Histocompatibility Antigens

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Abstract
The phytomitogens, concanavalin A (Con A)2 and phytohemagglutinin (PHA-M) have recently been reported to mask cellular histocompatibility (H-2) antigens on mouse lymphoid cells (1). The antiserum utilized in the masking experiments had antibodies directed against a number of H-2 specificities and was produced in mice after two sequential skin grafts and several (8 to 12) weekly injections of 25 × 106 spleen cells from an allogeneic host in the serum donor (2). It is possible that the phytomitogens may have interfered with the interaction between antibody and the H-2 antigenic sites, perhaps through steric hindrance from a non-H-2 antigen-antibody complex. The present study was designed to study the effects of PHA on the expression of human histocompatibility (HL-A) antigens on peripheral blood lymphocytes with monospecific antisera. We also studied the inhibitory effect of monospecific HL-A antisera on PHA stimulation of lymphocytes.

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