An Improvement of the Anderson Test by the Use of High Performance Liquid Chromatography

Abstract
The replacement of the N-benzyloxycarbonyl (Z) group in the Anderson peptide (Z–Gly–L/D–Phe–Gly–OEt) by Z–L-Ala and the subsequent separation of the diastereomers of the resulting tetrapeptide by reversed-phase HPLC offer a more convenient version with higher accuracy to the original Anderson test for studying racemization in peptide synthesis.