Lipid biosynthesis during the recovery of Salmonella typhimurium from thermal injury

Publisher Website
Google Scholar
Abstract
The heating of Salmonella typhimurium 7136 at 48C produced a sublethal injury. When injured cells were placed in fresh growth medium, they recovered within 3 h their normal tolerance to Levine Eosin Methylene Blue Agar containing 2% NaCl (EMBS). An isotope dilution assay demonstrated that 26.6% of the total lipid extracted from recovered cells was synthesized during the recovery period. Phospholipids synthesized during growth or recovery comprised about 90% of the total lipid. The concentrations of phosphatidyl ethanolamine and phosphatidyl glycerol synthesized during growth or recovery were similar; however, the amount of cardiolipin synthesized during recovery was greater than that obtained from normal cells. The fatty acid species synthesized during recovery were qualitatively similar but quantitatively dissimilar to the fatty acid profile of normal cells. The decreased concentrations of cyclopropane fatty acids with concomitant increases in their parent monoenoic acids gave presumptive evidence for the partial inactivation during injury of the enzyme cyclopropane fatty acid synthetase.

This publication has 0 references indexed in Scilit: