Carbon Assimilation Pathways during Growth of Pseudomonas AM1 on Methylamine and Pseudomonas MS on Methylamine and Trimethylsulphonium Salts

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Abstract
SUMMARY: A study was made of the incorporation of carbon from [14C]methylamine by cultures of Pseudomonas AMI growing on methylamine. The distribution of radioactivity within the non-volatile constituents of the ethanol-soluble fractions of the bacteria, after incubation for periods of up to 38 s, was analysed by chromato-graphy and autoradiography. Most of the radioactivity fixed at the earliest times of sampling appeared in serine, phosphorylated compounds and malate. These results contrasted with previous studies of the incorporation of labelled growth substrates by cultures of Pseudomonas MS growing on methylamine or trimethyl-sulphonium salts and correlated with the specific activities of hydroxypyruvate reductase, phosphoenolpyruvate carboxylase, N-methyl glutamate synthetase and γ-glutamylmethylamide synthetase in bacterial extracts. No hydroxypyruvate reductase was detectable in extracts of Pseudomonas MS grown either on methylamine or on trimethylsulphonium nitrate. The assimilation pathway for methylamine in Pseudomonas AMI appears essentially the same as that for methanol and different from that in Pseudomonas MS.

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