Abstract
Although carbon dioxide (CO2) is commonly used as an anesthetic for insects, its aftereffects are not completely known. Several investigators have observed effects ranging from the physiological to alterations of known behavior patterns (Brooks 1957, Edwards and Patton 1965). A side effect of CO2 anesthesia which is of direct interest to one of our research programs is that involving the mating and reproductive behavior of insects, especially moths. Janisch (1924) found that CO2 reduced both mating frequency and oviposition in the Mediterranean flour moth, Anagasta kuehniella (Zeller), females. Shorey (1964) found that CO2 reduced mating frequency of the cabbage looper, Trichoplusia ni (Hübner). Whisenant and Brady (1967) showed that Indian meal moth, Plodia interpunctella (Hübner), males after recovery from CO2 anesthetization were slow to respond to the sex attractant of the female and were also slow to mate.

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