THE ABSOLUTE LEVEL OF IgG Fc AND C3 RECEPTOR-POSTIVE T, B, AND NULL LEUKOCYTES WITHIN VARIOUS LYMPHOID COMPARTMENTS DURING ACUTE GRAFT-VERSUS-HOST DISEASE IN THE ADULT RAT

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Abstract
Acute lethal graft-vs.-host disease (GVHD) was produced in female (DA .times. LEW)F1 hybrid rats by injection 1.5 .times. 106/g body wt of semiallogeneic female LEW spleen and lymph node cells i.v. Control animals received the same dose of syngeneic cells. Animals inoculated with LEW cells died from acute GVHD by day 24. From 2-21 days after cell injection, the absolute number of IgG Fc and [complement component] C3 receptors on T, B and null cells was monitored in GVHD and control animals. Cell suspensions were prepared from the mesenteric and cervical lymph nodes and spleen, and mononuclear cell-separated preparations from peripheral blood. Such cell suspensions were stained indirectly in the presence of Staphylococcus protein A either with monoclonal antirat thymocyte antibody (w3/13) to label T cells (sT+) or antirat IgM (sIg+) to label B cells at 4 C and rosetted with either erythrocyte-antibody (EA, 7S) to simultaneously detect IgG Fc receptors or EA (19S) plus fresh mouse serum to detect C3 receptors. Sequential development of acute GVHD was divided into early (days 2-5), middle (days 7-10) and late (days 14-21) stages in comparing GVHD and control animals. Early GVHD was characterized by an absolute increase in sT+ cells in the peripheral blood, spleen and lymph nodes with a significant percentage also expressing IgG Fc receptors. The absolute number of null cells decreased during early GVHD. Middle GVHD was characterized by an absolute increase in sIg+ cells. From 58-91% sIg+ cells on day 7 expressed IgG Fc receptors, but only 41-45% expressed C3 receptors. Also evident during middle stage GVHD was a continued increase in T cells in most compartments until day 7 and then no change or even a decrease in the blood was observed. There continued to be a very significant increase in IgG Fc+ T cells within all compartment. Late GVHD exhibited a decrease in all T and B cells except those T cells with IgG Fc receptors. There was a 100-300% increase in null cells in all compartments, except blood; many of which did not express IgG Fc receptors.

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