A Practical Method for the Assay of Phenothiazines in Biologic Fluids

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Abstract
A specific method for estimating phenothiazine levels in blood and urine, which is practical for the routine clinical laboratory to perform, is described. The phenothiazine is extracted from the sample with heptane. It is reextracted into dilute HC1. Arsenic acid is then used to oxidize the phenothiazine to produce a color, the intensity of which is a measure of the phenothiazine level. The colors obtained follow the Beer-Lambert law. Absorbancemaxima and absorptivities vary with the substituents on the phenothiazine molecule. The locations of these peaks and factors for the relative intensities for the different phenothiazines are derived from experimental data. The phenothiazines studied and their absorbance maxima were: chlorpromazine 530, prochlorperazone (Compazine) 530, thiopropazate (Dartal) 530, triflupromazine (Vesprin) 505, thioridazine (Mellaril) 650, mepazine (Pacatal) 515, and promethazine (Phenergan) 520 nm.

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