Synthesis of Protein Complexes of Bis-indole Alkaloids from Catharanthus roseus

Abstract
Conjugation of vincristine (VCR) 1 and vinblastine (VBL) 2 to proteins through the C-10'' position of the "catharanthine moiety" is described. The procedure was applied to the production of immunogenic complexes of the drugs and bovine serum albumin (BSAl), and to the synthesis of an enzyme-linked tracer from VCR1 and alkaline phosphatase.
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