Two Replication Determinants of an Antibiotic-resistance Plasmid, pTB19, from a Thermophilic Bacillus
- 1 June 1984
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 130 (6) , 1399-1408
- https://doi.org/10.1099/00221287-130-6-1399
Abstract
Two different replication determinants were found on an antibiotic resistance plasmid, pTB19, from a thermophilic bacillus. One replication determinant (designated RepA) was functional only in Bacillus subtilis. whereas the other (designated RepB) functioned in both B. subtilis and Bacillus stearothermophilus. A deletion plasmid, pTB90, carrying the RepB derived from pTB19 coincidentally contained the specific 1-0 MDal Eco RI fragment of a cryptic plasmid pBSO2 from B. stearothermophilus. The presence of this 1.0 MDal Eco RI fragment in various deletion plasmids from pTB90 increased transformation frequencies for B. stearothermophilus 103 to 104 times and lowered plasmid copy numbers in the host strain to about one-tenth of those found for plasmids lacking this fragment.Keywords
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