Electrochemical Behaviour of a Lipid Modified Enzyme Electrode

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Abstract
The modification of the surface of a platinum electrode by coating with a layer of a lipid mixture (asolectin), allows the relative measurement of hydrogen peroxide in the presence of interfering analytes. The lipid-enzyme complex and the platinum amperometric sensor offer greater selectivity and extended stability of the resulting probe. Measurements of glucose with the glucose oxidase enzyme and detection of the liberated hydrogen peroxide have been performed as a model system. Linear response of the signal versus glucose concentration was observed in the range of glucose concentration 1.10−3 − 1.10−5 M with a response time of 20 s. The interferences of ascorbic acid, uric acid, iron (II), paracetamol, tyrosine and glutathion can be drastically minimized by appropriate adjustment of the amount of lipid contained in the biocatalyst layer.