Factors Influencing the Cell Type of Brain Tumors Induced in Dogs by Schmidt-Ruppin Rous Sarcoma Virus

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Abstract
Bacteria were eliminated from chicken sarcoma-extracted Schmidt-Ruppin Rous sarcoma virus (SR-RSV) and the virus was aseptically concentrated up to 80 fold with no loss of viral infectivity by differential centrifugation, sonication, and filtration. Suspension of the highly concentrated SR-RSV in 0.05 M sodium citrate buffer, pH 6.7, and storage at −70°C maintained brain tumorinducing potential of the virus suspension. After intracerebral inoculation of 0.1 ml of the highly concentrated 80 gm equivalents/ml SR-RSV into neonatal beagles, a 96% survival incidence and 100% brain tumor incidence (both sarcomas and gliomas) within 6 weeks was observed. In dose-response studies a graded host response with decreasing number of brain tumors per animal as well as decreasing numbers of inoculated animals with tumors was observed with decreasing SR-RSV doses. With a smaller (0.01 ml) inoculum volume of the most highly concentrated SR-RSV, brain inoculation site was the determinant of type of tumor induced; 7/10 dogs inoculated in the subependymal cell plate developed only gliomas, and all dogs in which tumors were induced after inoculation in the parietal subdural space (5/5) or the cerebellar vermis (4/5) developed sarcomas.

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