Mode of Replication of Plasmid λdv1

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Abstract
About 20 copies of plasmid ,λdv are perpetuated per host chromosome in Escherichia coli K12 cells. The mode of DNA replication of this plasmid λdv was studied, using the density-labeling technique followed by banding in CsCl. It was shown that all the copies of plasmid λdv are potentially capable of replicating roughly once a cell generation. Their replication occurs by random, that is a copy of the plasmid is taken out at random for replication from a pool, to which the two replicas resulting from replication are returned. Chloramphenicol did not inhibit the initiation of a new round of replication of the plasmid molecules, indicating that selection from the replication pool does not require concomitant protein synthesis.

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