The Rate and Extent of Anaerobic Decomposition of Sulfite Waste Liquor by Bacteria of Sea Water Mud

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Abstract
4 dilutions (1.5%, 5%, 16% and 26%) were incubated anaerobically at 9[degree], 21[degree] and 36[degree]C for 310 days; the evolved gases were collected, measured and analyzed. Analysis of the liquid itself for total solids, C and S content, pH, oxygen consumed, sugars and acids was also periodically made. The temp. of incubation and extent of dilution governed the rate of fermentation but did not appreciably affect the extent of decomposition. Organic acids were produced as intermediates prior to gas formation. Gasification was 25% of the carbon input. CO2 and CH4 were evolved, the ratio of the 2 gases varying with time; in the beginning CO2 predominated, at the end CH4 rose to 75-80% by volume; H was never found. Sulfites of the liquor were rapidly reduced to H2S, while sulfates of the sea-water were not attacked. The average heating value of the evolved gases was 649 B.t.u. per cu. ft. The liquor after completion of fermentation was stable, with a faint, not unpleasant odor.