Renin and Angiotensinase Content of the Kidney of Normal and Renal Hypertensive Rats.

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Abstract
The clipped (ischemic) and the contralateral (untouched) kidneys of rats which did and did not become hypertensive and of normal controls were extracted in salt solution and assayed for renin and angiotensinase activity. Renin was determined by two methods. Direct method consisted of a single injection into an assay rat prepared with urethane (1-1.5 g/kg I. P.) and pentolinium (3 mg/Kg S.C.). Indirect method required destruction of angiotensinase in the extract (pH 2.0 at 24[degree] for 60 min.), incubation with excess substrate, heat precipitation and injection of supernate into assay rat Results of both methods of assay were in substantial agreement Angiotensinase was measured as ability to destroy Va15 angiotensin II amide when incubated 2 hours at 37[degree] C. (Av.= 1000 u g inactivated per g of kidney). Results were as follows: The clipped (ischemic) kidney exhibited a higher renin concentration than the contralateral kidney whether the animal had become hypertensive or not. In either case the renin concentration of the clipped and of the undipped kidney was less than in control animals. Renal angiotensinase concentration under the various experimental conditions was not altered except for a small reduction (P greater than .1) in both kidneys of the rats which were operated upon but did not become hypertensive.

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