Biochemistry of fluoroacetate poisoning: the effect of fluorocitrate on purified aconitase

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Abstract
The competitive inhibition of aconitase by natural fluorocitrate was established. On the other hand, synthetic fluorocitrate was shown to inhibit aconitase in both a competitive and an apparent irreversible fashion. The dissociation constants of the aconitase-fluorocitrate complexes for both the natural and synthetic fluoro-citrates were measured. The affinity of aconitase for the synthetic compound is much greater than for the natural compound. The possible reasons have been discussed for the difference between the 2 preparations of fluorocitrate in inhibiting the isolated aconitase and also for the difference between the action of these compounds and the isolated aconitase system as compared to their action in vivo and on the kidney-particle system.