The Assay of Clozapine and N-Desmethylclozapine in Human Plasma by High-Performance Liquid Chromatography
- 1 January 1991
- journal article
- Published by Wolters Kluwer Health in Therapeutic Drug Monitoring
- Vol. 13 (1) , 69-72
- https://doi.org/10.1097/00007691-199101000-00010
Abstract
A high performance liquid chromatographic method for the simultaneous quantitation of clozapine--a member of the dibenzodiazepine class of antipsychotic drugs--and its reduced metabolite in human plasma has been developed. Clozapine and N-desmethylclozapine are concentrated from blood samples by liquid-liquid extraction, followed by reverse-phase liquid chromatography. Accuracy of the determination is ensured by application of an internal standard, protriptyline, which is closely related to clozapine. A detection limit of 15 ng/mL for clozapine and 30 ng/ml for N-desmethylclozapine was achieved.Keywords
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