Cloning and nucleotide sequence of mouse immunoglobulin epsilon chain cDNA.

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Abstract
Complementary DNA (cDNA) corresponding to mouse IgE heavy (.epsilon.) chain mRNA was cloned from mouse IgE-secreting hybridoma cells. A clone containing the .epsilon. cDNA insert was identified by hybridization to .epsilon. mRNA and subsequent translation in vitro to unprocessed .epsilon. chain reactive with anti-mouse IgE antibodies. This clone was used to select 20 other .epsilon. cDNA clones by colony hybridization. The clone containing the longest insert was selected and the .epsilon. cDNA insert was subjected to sequence analysis. The determined sequence is 1279 nucleotides long and contains the coding regions for part of the constant region (C.epsilon.) 1 and all of the C.epsilon.2, C.epsilon.3, and C.epsilon.4 domains and the entire 3'' untranslated region of .epsilon. mRNA. When the amino acid sequence determined from the nucleotide sequence is compared to that of human .epsilon. chain, significant homologies between corresponding domains of the 2 .epsilon. chains are found, including conservations in cysteine and tryptophan residues and carbohydrate attachment sites.