VARIATIONS OF BLOOD-LYMPHOCYTES DURING WORK STUDIED BY CELL-SURFACE MARKERS, DNA-SYNTHESIS AND CYTOTOXICITY

  • 1 January 1976
    • journal article
    • research article
    • Vol. 24  (2) , 328-335
Abstract
Highly purified peripheral blood lymphocytes from healthy individuals were obtained from samples collected before and after a standardized bicycle ergometer test. The physical activity resulted in a marked increase of circulating lymphocytes. The proportion of T [thymus-derived] lymphocytes, estimated as cells forming sheep red blood cell rosettes after incubation in the cold, decreased; a corresponding increase of cells with receptors for C3 [the 3rd complement component], IgG [immunoglobulin G]-Fc or surface Ig was noted. After work, an increase of cells simultaneously reacting with cell surface markers usually designed as T- or B[bone marrow-derived]-cell markers occurred. The reactivity of lymphocytes collected after work in response to Con A [concanavalin A], PHA [phytohemagglutinin], PWM [pokeweed mitogen], and PPD [purified protein derivative] was impaired; the slight response to LPS [lipopolysaccharide] [Escherichia coli] was unchanged. The K[antibody-dependent killer]-cell cytotoxicity of lymphocytes collected after work increased. Physical activity apparently leads to the mobilization of lymphocytes from as yet undetermined sites.