A Two Step Synthesis of the Nucleoside Q Precursor 2-Amino-5-cyanopyrrolo[2,3-d]pyrimidin 4-one (PreQo)

Abstract
PreQo (1) is prepared in a 70 % yield by the condensation of chloro(formyl)acetonitrile (2) with 2,6-diaminopyrimidin-4-one (3), and subsequently converted to its amide derivative 5. Isolation of the intermediate 4 in the condensation reaction was also observed giving further insight into the proposed mechanism.

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