Differentiation of the anterior latissimus dorsi muscle of the chicken examined by anti‐myosin monoclonal antibodies

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Abstract
Two new monoclonal antibodies (McAbs), ALD‐180 and ALD‐88, produced against the myosin of the slow anterior latissimus dorsi (ALD) muscle of the chicken are described. Their specificity for myosin heavy chain (MHC) was established by radioimmunoassay, immunoautoradiography, and immunofluorescence. They were used in conjunction with McAbs MF‐14 and MF‐30 (which have been characterized previously to be directed against MHC of the fast skeletal muscle) to examine the developmental changes of the chicken ALD muscle. At the 16‐day embryonic, early posthatch, and adult stages the ALD muscle fibers differed in their reaction pattern with the McAbs: at the embryonic stage all fibers reacted strongly with ALD‐180 and weakly with ALD‐88 and MF‐30; at the early posthatch stage there was a checkerboard pattern with many fibers not reacting with any of these three McAbs; and at the adult stage all fibers reacted strongly with ALD‐180 and ALD‐88 and weakly with MF‐30. The MF‐14 antibody did not react with ALD muscle at any developmental stage. The mature pattern of immunoreactivity of the ALD muscle fibers with the antibodies was established only after 9 weeks posthatch, and during this 9‐week period the immunofluorescence changes were nonsynchronous. Based on immunocytochemical evidence of changes in myosin isoform expression, this study clearly demonstrates a distinctive neonatal (early posthatch) stage in the development of the chicken slow muscle.