T4 DNA ligase can seal a nick in double-stranded DNA limited by a 5′-phosphoryiated base-free deoxyribose residue

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Abstract
The 5′ AP endodeoxyribonucleases hydrolyze the phosphodiester bond 5′ to AP (apurinic or apyrimidinic) sites in double-stranded DNA leaving 3′-OH and 5′-phosphate ends. These nicks are sealed by T4 DNA ligase although the 5′-phosphate end belongs to a base-free deoxyribose.