Inhibition of Ovulation by Synthetic Samples of the Tetrapeptide Originally Isolated from Hamster Embryos1

Publisher Website
Google Scholar
Abstract
The tetrapeptide, H-thr-pro-arg-lys-OH, has been synthesized in 5 laboratories. The natural tetrapeptide, extracted from hamster embryos, prevents maturation of Graafian follicles and the subsequent appearance of postovulatory structures when administered daily during 1 estrous cycle prior to the anticipated ovulation. The synthetic compounds, increased s.c., were tested for the same biological effect in both hamsters and rats. Only the compound prepared by Dr. R.W. Roeske and K.U. Prasad (Indiana) and that prepared by CalBioChem were biologically effective. The Roeske and Prasad sample was administered orally to hamsters and was effective. The basis for the presence or absence of biological activity in the 5 samples tested was correlated with the apparent proportion of active tetrapeptide per sample using isolation of components on Sephadex G-10 as the main technique.

This publication has 1 reference indexed in Scilit: