BLOOD AMINO ACID STUDIES: I. A MIGROMETHOD FOR THE ESTIMATION OF FREE LYSINE, METHIONINE, AND THREONINE
- 1 November 1961
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 39 (11) , 1669-1674
- https://doi.org/10.1139/o61-184
Abstract
By a modification of conventional methods of microbiological assay it was possible to determine the free lysine, methionine, and threonine in 0.2 ml of rat plasma. The small amounts of lactic acid produced in the microassay were estimated photometrically by measurement of the color change of bromthymol blue. Repeated analysis of samples on different days indicated no significant effect of days and the confidence limits for the assays were approximately ± 6% (p = 0.05). Mixtures of foods containing bread and various supplementary proteins were fed to young male rats for 24 hours and then plasma free lysine and methionine were measured. There was a direct relation between plasma free lysine levels and the lysine content of the diet. The method permits the accurate estimation of amino acids in blood of individual rats or in small samples of human plasma.This publication has 6 references indexed in Scilit:
- Levels of lysine and methionine in portal blood of rats following protein feedingArchives of Biochemistry and Biophysics, 1960
- Relationship between plasma amino acids and composition of the ingested proteinArchives of Biochemistry and Biophysics, 1959
- Effects of Fasting on Blood Non-Protein Amino Acids in HumansJournal of Nutrition, 1955