Analysis of hexosaminitol-containing disaccharide alditols from rat brain glycoproteins and gangliosides asO-trimethylsilyl derivatives by gas chromatography mass spectrometry

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Abstract
The O-trimethylsilyl derivatives of five reference disaccharide alditols composed of a N-acetylhexosaminitol substituted at C-3, C-4 or C-6 with a hexose moiety were studied by gas chromatography mass spectrometry. The data were used in the determination of the linkage in disaccharide alditols derived from rat brain glycoproteins and gangliosides. The O-glycosidically linked carbohydrate units of brain glycoproteins contained two oligosaccharides, β-galactosyl-(1 → 3)-N-acetylgalactosaminitol and α-galactosyl-(1 → 3)-N-acetylgalacto-saminitol, whereas only the latter was obtained from brain gangliosides after partial acid hydrolysis and reduction.