An assessment of the calcium content of rat liver mitochondria in vivo

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Abstract
The effect of systematically altering the isolation conditions on the total Ca content of mitochondria isolated from perfused rat liver was examined. Under most isolation conditions, significant redistributions of mitochondrial calcium occurred resulting in up to 5-fold changes of the total Ca content. Mitochondrial Ca2+ flux inhibitors such as Ruthenium Red and nupercaine were only partially effective in inhibiting such redistributions. The total Ca content of rat liver mitochondria in situ may approximate 2 nmol .cntdot. (mg of protein)-1.