In Vitro Differentiation of a Wild Peanut, Arachis villosulicarpa Hoehne1,,2

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Abstract
Immature leaflets 3 to 5 mm in length from Arachis villosulicarpa Hoehne seedlings were cultured in vitro on a medium consisting of the major and minor salts of Murashige and Skoog (MS), Gamborg's B5 vitamins, 20 g/L sucrose, 0.8% Difco agar, 4.44 μM N-6 benzyladenine (BA) and 5.37 μM napthaleneacetic acid (NAA). Hard callus with many shoot primordia formed on all leaflets within 4 to 6 weeks. Calli with primordia were further cultured for plant formation on 150 other media (MS major salts at 1/2 or 1/4 strength, full strength minor salts and vitamins, sucrose at 5 or 10 g/L, BA and NAA at 0 to 8 μM). Reduced concentrations of major salts with a high ratio of BA to NAA enhanced the formation of shoot primordia. Roots differentiated on media with 1/4 strength major salts, no BA, and 6 to 8 μM NAA and 5 g/L sucrose. Shoots differentiated from the branch points of roots after 2 to 4 months of culture. Plants were obtained via shoot primordia from callus and from the adventitious shoots from roots.

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