Uptake and metabolism of androgen by the human epididymis in vitro

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Abstract
Summary. Uptake and metabolism of (3H)testosterone, mainly to 5\g=a\-dihydro- testosterone (5\g=a\-DHT)and 5\g=a\-androstanediolwere higher in the caput than in the cauda epididymidis in vitro. The metabolites represented 57, 49 and 47% of the total radioactivity in the caput, corpus and cauda epididymidis, respectively; subcellular distributions of the metabolites in each segment showed 67% of total radioactivity in cytosol and 18% in the nuclei. In both fractions, the amount of 5\g=a\-DHTwas greater than that of androstanediol while the reverse was true for the mitochondria and microsomes. The distribution of 5\g=a\-reductaseactivity in subcellular fractions was similar to that of the microsomal marker enzyme NADPH: cytochrome C reductase, whilst 3\g=a\-hydroxyst eroid dehydrogenase was found mainly in the cytosol. Maximal 5\g=a\-reductaseactivity was at pH 5\m=.\3,apparent Km values in the microsomal and nuclear fractions were 1\m=.\65\m=+-\0\m=.\7and 1\m=.\75\m=+-\0\m=.\36\m=x\10\m=-\6 M respectively, and the

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