Abstract
LD50 (lethal dose 50) temperature estimates were utilized to study cold acclimation in three winter and one spring wheat (Triticum aestivum L.) cultivars grown under natural and controlled environments. Highly significant differences were found among cultivars for both rate and maximum level of cold acclimation with the acclimation patterns being similar for both environments. The advantages and limitations to the use of lethal dose temperature ratings in cold hardiness studies are discussed.