LACK OF DIFFERENCE IN TESTOSTERONE METABOLISM BETWEEN CULTURED SKIN FIBROBLASTS OF HUMAN ADULT MALES AND FEMALES

Abstract
The metabolism of testosterone by cultured human fibroblasts derived from skin in the deltoid area of 4 adult males and 6 adult females was investigated. Testosterone-14C was incubated with monolayer cultures for 48 hr and the following labeled metabolites were identified: 5α-androstan-3α,17β-diol, testosterone, androsterone, 5α-dihydrotestosterone, AlΔ4-androstenedione and androstandione. The amount of testosterone metabolized by the 17β-OH versus the 17=0 pathway was expressed as the 17β-0H/17=0 ratio. No differences in testosterone metabolism between fibroblasts from adult males and females were found. This is contrary to the report of Shanies et al (1).

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