PEROXIDASE‐GENERATED TRIPLET INDOLE‐3‐ALDEHYDE ADDS TO URIDINE BASES AND EXCITES THE 4‐THIOURIDINE GROUP IN t‐RNAPhe
- 1 July 1982
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 36 (1) , 21-24
- https://doi.org/10.1111/j.1751-1097.1982.tb04334.x
Abstract
Triplet indole‐3‐aldehyde generated by the peroxidase catalyzed aerobic oxidation of indole‐3‐acetic acid adds to t‐RNAphe with an efficiency which can reach 20% of the theoretical value based upon the substrate. The reaction occurs with uridine groups and is of the Paterno‐Büchi type. Magnesium ions reduced addition and increased the sensitized emission from the 4‐thiouridine group. Our results, coupled with available biological data obtained with selectively labeled indole‐3‐acetic acid, support the hypothesis that formation of triplet indole‐3‐aldehyde is at the root of the biological effects of indole‐3‐acetic acid.Keywords
This publication has 18 references indexed in Scilit:
- Transfer RNA-Peroxidase InteractionPlant Physiology, 1980
- Generation and transfer of triplet energy in enzymic systemsAccounts of Chemical Research, 1980
- Photobiochemistry in the DarkPublished by Springer Nature ,1980
- Photobinding of 8-methoxypsoralen to transfer RNA and 5-fluorouracil-enriched transfer RNABiochemistry, 1978
- CHEMILUMINESCENCE FROM THE OXIDATION OF AUXIN DERIVATIVESPhotochemistry and Photobiology, 1976
- 4-Thiouridine, a built-in probe for structural changes in transfer RNABiochemistry, 1976
- Use of photochemically induced crosslinking as a conformational probe of the tertiary structure of certain regions in transfer ribonucleic acidBiochemistry, 1973
- Studies on soluble RNA binding indoleacetic acid in etiolated mung bean hypocotyl sectionsPlant and Cell Physiology, 1972
- Introduction of an intramolecular flourescent pobe in E. coli tRNAVal1FEBS Letters, 1971
- Labeled Indole-Macromolecular Conjugates from Growing Stems Supplied with Labeled Indoleacetic AcidPlant Physiology, 1971