PREPARATION PROCEDURE FOR CEREBROSPINAL-FLUID THAT YIELDS CYTOLOGIC SAMPLES SUITABLE FOR ALL TYPES OF STAINING, INCLUDING IMMUNOLOGICAL AND ENZYMATIC METHODS

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  • 1 May 1987
    • journal article
    • research article
    • Vol. 31  (3) , 374-376
Abstract
A new method for the cytologic investigation of the cerebrospinal fluid is described. Preparation of the cells is done by successive centrifugation, resuspension of the cells in a protein-enriched medium and cytocentrifugation. The method provided both good cell yields and morphologic preservation. All stainings, including enzymecytochemical and immunocytochemical, could be performed on the specimens. The cell-free fluid from the first centrifugation is also available for use in other studies.