IL–1β protein in human skin lymph does not discriminate allergic from irritant contact dermatitis

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Abstract
Recent data suggest a key rôle for IL‐1β in the induction phase of allergic contact dermatitis. In the present study, (he protein levels of IL‐1β were measured in skin lymph derived from normal untreated skin as well as from irritant and allergic (induction and elicitation phase) contact dermatitis. IL‐1β increased in the course of both types of contact dermatitis, displaying the highest levels in irritant CD. Using a reverse transcriptase‐polymerase chain reaction, low signal strength of IL‐1β mRNA was demonstrated in lymph cells derived from normal skin and allergic CD. In lymph cells collected 2 × daily during the induction phase of allergic contact dermatitis, no upregulation of the IL‐1β mRNA signal was found. Isolated CD 1a+ lymph cells derived from normal skin us welt as from the induction and elicitation phase of allergic contact dermatitis did not express IL‐1β mRNA. Our results demonstrate that in human skin lymph, the IL‐1β p profiles do not discriminate between irritant and allergic contact dermatitis and that besides resident epidermal and dermal cells, circulating lymph cells may also contribute to 1L‐1β protein production.